...the Tabula Sapiens Consortium discovered that endothelial cells from lung, heart, uterus, liver, pancreas, fat, and muscle exhibit the most distinct transcriptional signatures, suggesting highly specialized functions, whereas endothelial cells from the thymus, vasculature, prostate, and eye resemble one another. The pan-tissue approach led to the discovery of SLC14A1 (solute carrier family 14 member 1) as a marker for heart endothelial cells, likely reflecting specialized metabolism in cardiac blood vessels. Eraslan et al. also found rare cell types, such as neuroendocrine cells in the prostate and enteric neurons in the esophagus. Additionally, the corroborative use of both high-throughput 10X and full-length SMART-seq2 single-cell transcriptome data allowed the quantification of splicing isoform usage at the single-cell level, thereby revealing differential exon usage patterns for genes, including MYL6 (myosin light chain 6) and CD47, in different cell-type compartments.
Friday, May 13, 2022
The tabula sapiens consortium - mapping cell types in the human body
It is hard to keep up with the mind boggling advances that pop up in almost every issue of Science Magazine. In a perspective article Liu and Zhang describe the findings of the “Tabula Sapiens Consortium” that has now provided a molecular reference atlas for more than 400 cell types of the human body by measuring the messenger RNA molecules in each of nearly 500,000 cells from 24 tissues and organs. Multiple laboratories used single-cell transcriptomics to measure the messenger RNA molecules in each of nearly 500,000 cells from 24 tissues and organs Here is a single clip summary clip from Liu and Zhang: